The Leica STED-CW is the complementary device to the confocal microscope providing nanoscopic research level. The device allows to study living cells with the dot pattern of 512 x 512 dots, with the up to 18 frames/sec speed and with the resolution up to 80 nm; to study living cells a number of fluorescen proteins could be used, such as Citrine, YFP, etc.; to study fixed material with the resolution up to 80 nm conventional fluorescent dyes such as Alexa 488, FITC and Oregon Green could be used; to use singe molecule detection technique for the reduction of the excitation spot in the lateral plane up to 80 nm. For more information visit