Optical microscopy as no other method has been able to demonstrate for biology researches the relationship between available research methods and scientific results obtained. The ability to explore the biological object in vivo, directly observe the processes, this all makes this method irreplaceable in a lot of researches. The resource center is represented by modern microscopic technique including confocal and two-photon microscopy, super resolution techniques, special optical methods such as the detection of individual fluorescent molecules, determining the concentration of analytes in living cells, the determination of the rate of diffusion of molecules of nutrients, etc. An important component is the availability of all necessary equipment for work with living cells including micromanipulation, the massive research and cell sorting by flow cytometer sorter. In addition to the optical equipment in research center, there are modern software for morphometric and image processing.
Instruments
QuantiFluor (Promega) and Qubit 2.0 (Invitrogen) fluorometers
Principle
QuantiFluor (Promega) and Qubit 2.0 (Invitrogen) fluorometers are used for the quantitation of DNA, RNA, and protein by using the highly sensitive and accurate fluorescence-based quantitation assays. Use of the state-of-the-art dyes selective for dsDNA, RNA, and protein minimizes the effects of contaminants in your sample that affect the quantitation. Uses the QuantiFluor and Qubit assays that contain advanced dyes that only fluoresce when bound to DNA, RNA, or protein. This specificity allows you to get very accurate results because these technologiesy only reports the concentration of the molecule of interest, not contaminants.
Flow cytometry technique provides analysis of multiple (both physical and chemical) characteristics of single cells and particles flowing in single row in a stream of fluid. The technique is based on the registration of scattering and emitted light from fluorescently labeled markers. Light scattering at different angles can distinguish differences in size and internal complexity, whereas light emitted from fluorescently labeled antibodies can identify a wide array of cell surface and cytoplasmic antigens. The information obtained is both qualitative and quantitative. Flow cytometry technique is mainly used for immunophenotyping of a variety of specimens, including whole blood, bone marrow, serous cavity fluids, cerebrospinal fluid, urine, and solid tissues.
Metabolomics is a field of life science about chemical processes involving metabolites. The combination of these metabolites is called the metabolome
This is one of the most perspective direction among molecular –omics. Metobolomic today is not only postgenomic analysis, but full value direction. Metabolic profile can be an alternative view to biological systems, different from proteomic genomic and transcriptomic points of view. Metabolome is one of the most changeable systems in biology systems. That’s why metabolomics research is a brilliant way for ecological, functional genomics, toxicology, medicine and many other areas.
