The Applied Biosystems 7500 Fast Real-Time PCR System uses fluorescent- based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point and dissociation-curve analysis. The Applied Biosystems 7500 Fast Real-Time PCR System allows the user to perform high-speed thermal cycling giving run times for quantitative real-time PCR applications (such as relative quantitation) in fewer than 40 minutes.
Method description: http://find.lifetechnologies.com/Global/FileLib/StepOne_Test/Intro-to-Gene-Expression.pdf
