The Quantus fluorimeter is an instrument for measuring the concentration of nucleic acids. The device is used with a set of reagents Promega QuantiFluor® Dye Systems.
Before using the appliance for the first time, you need to calibrate the device, also you should calibrate before you start using each new set of reagents.
Materials:
0.5 PCR tube (thin-walled PCR tubes Axygen PCR-05-C);
20X TE ((pH 7.5) buffer;
dsDNA QuantiFluor®/ RNA QuantiFluor®.
Measurement of concentration of samples
- 1. In 0.5 PCR test tubes add 100 µl of QuantiFluor®, the necessary volume of samples (1-100 µl) and to increase the volume in the tubes to 200 µl of 1X TE.
- 2. Select the necessary Protocol, the volume of the analyzed sample and unit of concentration on the instrument display.
- 3. Put the sample into the instrument and close the lid. The concentration of the sample is shown in the display.
