The MiSeq personal sequencing system enables researchers to go from sample to analyzed data in as little as eight hours with a revolutionary workflow and unmatched accuracy. Capable of generating up to 7.0 Gb per run, MiSeq is the only next-generation sequencer that integrates amplification, sequencing, and data analysis in a single instrument.
Manufacturer: Illumina, USA.
Web site: http://www.illumina.com/systems/miseq.html.
Main characteristics:
· Cluster Generation and Sequencing
|
MiSeq Reagent Kit v2 |
||
|
Read Length |
Total Time* |
Output |
|
1 × 36 bp |
~4 hrs |
540-610 Mb |
|
2 × 25 bp |
~5.5 hrs |
750-850 Mb |
|
2 × 150 bp |
~24 hrs |
4.5-5.1 Gb |
|
2 × 250 bp |
~39 hrs |
7.5-8.5 Gb |
|
MiSeq Reagent Kit v3 |
||
|
Read Length |
Total Time* |
Output |
|
2 × 75 bp |
~21 hrs |
3.3-3.8 Gb |
|
2 × 300 bp |
~56 hrs |
13.2-15 Gb |
· Reads Passing Filter
|
MiSeq Reagent Kit v2 |
|
|
Single Reads |
12-15 M |
|
Paired-End Reads |
24-30 M |
|
MiSeq Reagent Kit v3 |
|
|
Single Reads |
22-25 M |
|
Paired-End Reads |
44-50 M |
· Quality Scores
|
MiSeq Reagent Kit v2 |
|
> 90% bases higher than Q30 at 1 x 36 bp |
|
> 90% bases higher than Q30 at 2 x 25 bp |
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> 80% bases higher than Q30 at 2 x 150 bp |
|
> 75% bases higher than Q30 at 2 x 250 bp |
|
MiSeq Reagent Kit v3 |
|
> 85% bases higher than Q30 at 2 x 75 bp |
|
> 70% bases higher than Q30 at 2 x 300 bp |
Contact our staff for the details about the system and its availability.
