Flow cytometry includes complex of methods:

  1. 1. Cell analysis:
  • -Identification of populations and subpopulations of cells by analysis of cell surface and cytoplasmic antigens
  • -Analysis of functional conditions of cells (mitochondrial potential, intracellular Ca2+, pH) using ratiometric dyes (FDA, DiOC1, JC -1, etc.)
  • -Analysis of functional conditions of cells using specific antibodies against phosphorylated and dephosphorylated forms of proteins
  • -Multiparametric analysis of different samples
  • -Cell cycle analysis including DNA analysis of live and fixated cells
  • -Kinetic Measurements of cellular processes (cell membrane disruption, enzyme activity)
  • -Analysis of apoptotic cell death mechanisms and stages
  • -Flow cytometry analysis of phagocytosis
  • -Molecular-genetic analysis including of single chromosome analysis
  • -Chromosome karyotype study using fluorescent probes
  1. 2. Cell sorting:
  • -Gating- based sorting up to 4 populations simultaneously
  • -Rare cell sorting
  • -Single cell sorting into cultural or PCR plates 

Literature and sources:

Michael Brown and Carl Wittwer. Flow Cytometry: Principles and Clinical Applications in Hematology. Clinical Chemistry. August 2000 vol. 46 no. 8 1221-1229