This protocol explains how to prepare libraries from genomic DNA (gDNA) for subsequent cluster generation and DNA sequencing using the reagents provided inthe Illumina Nextera® DNA Library Preparation Kit.

Method description: http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_nextera/nexteradna/nextera-dna-library-prep-reference-guide-15027987-01.pdf.

The Applied Biosystems 7500 Fast Real-Time PCR System uses fluorescent- based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point  and dissociation-curve analysis. The Applied Biosystems 7500 Fast Real-Time PCR System allows the user to perform high-speed thermal cycling giving run times for quantitative real-time PCR applications (such as relative quantitation) in fewer than 40 minutes. 

Method description: https://tools.thermofisher.com/content/sfs/manuals/4387783c.pdf

The Applied Biosystems 7500 Fast Real-Time PCR System uses fluorescent- based PCR chemistries to provide quantitative detection of nucleic acid sequences using real-time analysis and qualitative detection of nucleic acid sequences using end-point  and dissociation-curve analysis. The Applied Biosystems 7500 Fast Real-Time PCR System allows the user to perform high-speed thermal cycling giving run times for quantitative real-time PCR applications (such as relative quantitation) in fewer than 40 minutes. 

Method description: http://find.lifetechnologies.com/Global/FileLib/StepOne_Test/Intro-to-Gene-Expression.pdf

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments: https://www.qiagen.com/ru/resources/resourcedetail?id=e9f6cb28-0ae7-400d-b0de-19e734798aed&lang=en.

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments: https://www.qiagen.com/ru/resources/resourcedetail?id=f6158498-a857-4a2f-b40b-569fba3793e2&lang=en.

This appliance is intended for cryopreservation (freezing) of various types of biological samples down to low and ultra-low temperatures. Can be used in the creation of biobanks of different types of samples (blood, cells, tissues).

The freezing of samples

  • Materials – liquid nitrogen;

2D cryovials FluidX: 0.3 ml, 0.7 ml, 1.0 ml, 0.75 ml, 1.3 ml.