The MiSeq personal sequencing system enables researchers to go from sample to analyzed data in as little as eight hours with a revolutionary workflow and unmatched accuracy. Capable of generating up to 7.0 Gb per run, MiSeq is the only next-generation sequencer that integrates  amplification, sequencing, and data analysis in a single instrument.


  

Manufacturer: Illumina, USA

Status: commissioned 

Web site: http://www.illumina.com/systems/miseq.html

1. Main characteristics:

·         Cluster Generation and Sequencing

MiSeq Reagent Kit v2

Read Length

Total Time*

Output

1 × 36 bp

~4 hrs

540-610 Mb

2 × 25 bp

~5.5 hrs

750-850 Mb

2 × 150 bp

~24 hrs

4.5-5.1 Gb

2 × 250 bp

~39 hrs

7.5-8.5 Gb

MiSeq Reagent Kit v3

Read Length

Total Time*

Output

2 × 75 bp

~21 hrs

3.3-3.8 Gb

2 × 300 bp

~56 hrs

13.2-15 Gb

 

·         Reads Passing Filter

MiSeq Reagent Kit v2

Single Reads

12-15 M

Paired-End Reads

24-30 M

 

MiSeq Reagent Kit v3

Single Reads

22-25 M

Paired-End Reads

44-50 M

 

·         Quality Scores

MiSeq Reagent Kit v2

> 90% bases higher than Q30 at 1 x 36 bp

> 90% bases higher than Q30 at 2 x 25 bp

> 80% bases higher than Q30 at 2 x 150 bp

> 75% bases higher than Q30 at 2 x 250 bp

 

MiSeq Reagent Kit v3

> 85% bases higher than Q30 at 2 x 75 bp

> 70% bases higher than Q30 at 2 x 300 bp

 

2. Contact our staff for the details about the system and its availability